Enemies are a very present element in VVVVVV. They are very similar to Spikes, as when the player touches them, Viridian is killed and respawns at the last checkpoint. However, Enemies move by a pattern or by the two extremes of the screen. The most common enemy is the 2x2 box shaped enemy, but there are various types of enemies with various sizes. In Editor Mode, they can move down, up, left and right. Enemies can have different speeds and sprites.
- Enemies don't have a specific stage, though they're used quite a lot in The Final Level
- Even though enemies have various sizes, only the 2x2 enemies can be used in the level editor.